Do you lose Serana if you don't become a vampire?

No, you do not lose Serana if you don't become a vampire; she will still join you and remain a follower if you side with the Dawnguard, and she can even be convinced to cure her vampirism at the end of the DLC, allowing you to stay human and keep her as a companion. She functions as your guide and follower regardless of your choice, either at Fort Dawnguard (if you side with them) or Castle Volkihar (if you side with vampires).
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Can you still get Serana if you don't become a vampire?

No, you do not have to become a vampire to get Serana; she becomes your follower regardless of whether you side with the Dawnguard or the Volkihar vampires, as she's essential to the main questline, but becoming a Vampire Lord offers different quest progression and benefits, while siding with Dawnguard allows you to cure her vampirism later, as explained in this YouTube video from a Skyrim playthrough. She'll join you at Fort Dawnguard if you're Dawnguard, or at Castle Volkihar if you're a vampire, guiding you through the story. 
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Can you marry Serana after she's cured?

No, you cannot marry Serana in the base Skyrim game, even after curing her vampirism, as she doesn't have the necessary dialogue or mechanics for marriage; however, you can marry her by using various player-made mods (like Serana Dialogue Add-On, Amorous Adventures, or Marry Me Serana) that add this functionality. Her character, due to past trauma, is naturally hesitant about marriage in the vanilla game, but mods can create a more in-depth romance and marriage path.
 
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Can you keep Serana as a follower if you join Dawnguard?

Yes, you absolutely can get Serana as a follower if you join the Dawnguard; she's a core companion for the entire Dawnguard DLC questline, regardless of which faction (Dawnguard or Vampires) you ultimately side with, and remains a permanent follower after completion if you choose. She'll follow you through most of the main story as an essential guide, and after the questline, she'll stay at Fort Dawnguard (or Castle Volkihar if you chose vampires) and can be recruited indefinitely. 
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Can you turn Serana back into a human?

Yes, you can turn Serana human in Skyrim by siding with the Dawnguard in the DLC and convincing her after defeating her father, Harkon, but you must choose specific dialogue options during the questline to make it possible; if you ask her about a cure too early or insult her, she'll refuse permanently, losing the ability to be cured later. Once cured, she loses vampiric abilities, and you lose the ability to become a Vampire Lord.
 
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Skyrim theory Why you should NEVER marry Serana explained

How many times will Serana turn you into a vampire?

Serana can turn you into a Vampire Lord an unlimited number of times, provided you've completed the Dawnguard questline and haven't persuaded her to cure herself. After the main quest, you can simply ask her to transform you as often as you like, but you'll need to get cured by Harkon or a priest to interact normally with the Dawnguard again. 
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Should I become a vampire or let Serana Soul trap me?

For most players, letting Serana soul trap you (partial soul trap) is the better choice, as it's a minor debuff in the Soul Cairn you can recover from, while becoming a Vampire Lord locks you into vampirism (unless cured), potentially hindering the Dawnguard questline and causing issues if you want to remain mortal or a Werewolf; you can always become a Vampire Lord later if you want, even after the main quest. 
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How many times can Serana turn you?

Serana can turn you back into a Vampire Lord an infinite number of times, but only after the entire Dawnguard questline is complete. She loses this ability if you persuade her to cure herself of vampirism. When you are a vampire, the Dawnguard will refuse to interact with you except to tell you how to cure yourself.
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Is it better to side with vampires or Dawnguard?

Choosing between Dawnguard or Vampire in Skyrim's DLC depends on your playstyle: Dawnguard offers powerful crossbows, unique sun-based spells, trolls, and a focus on traditional vampire hunting, while Vampires provide the potent Vampire Lord form with unique powers, vampire-specific jewelry, and a darker roleplaying experience, though becoming a vampire early can alienate Dawnguard members and vendors. You can even join the Vampires later as a Dawnguard member for a mix of rewards, but expect bugs with Dawnguard interactions.
 
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Can you marry Aela as a vampire?

To marry her you have to finish the companions quest line. Since that questline turns you into a werewolf you won't be able to stay a vampire.
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How old is Serana?

Serana from The Elder Scrolls V: Skyrim is over 1,000 years old, having been sealed in Dimhollow Crypt for centuries, though her exact age when turned into a vampire (around the late Merethic or early First Era) isn't specified, she appears as a young adult, likely in her 20s or 30s, due to her vampiric nature stopping her aging process.
 
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Who is the best female to marry in Skyrim?

There's no single "best" wife in Skyrim, as it depends on player preference, but Aela the Huntress, Mjoll the Lioness, and Ysolda are consistently top choices for their skills, personalities, or convenience as merchants, with Aela often favored for her warrior skills and werewolf connection, while Ysolda offers easy gold. Other popular choices include Borgakh the Steel Heart for an adventurous Orc or Lydia if you want a loyal housecarl.
 
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What happens if Serana cures herself?

If you cure Serana in Skyrim's Dawnguard DLC, she becomes mortal, loses her Vampire Lord powers and bloodcursed arrows, and her eyes change, but she retains much of her personality, complaining about the sun and still disliking temples, making it a choice for roleplaying rather than major gameplay benefit, though she can then be healed by normal Restoration spells.
 
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What race is Serana?

Serana is a Nord who became a pure-blood Vampire, known as one of the "Daughters of Coldharbour," in The Elder Scrolls V: Skyrim's Dawnguard DLC. While her race is technically Nord, her existence as an ancient, powerful vampire makes her distinct, and she's a central figure in the questline, often appearing in her hooded, vampiric form.
 
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Will the Dawnguard hate you if you become a vampire?

Before you join, showing up as a vamp (no matter the stage) will make them hostile. If you've already joined, they aren't going to attack you, but you'll be unable to continue their quest or doing any jobs for them.
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What is the level cap for Serana?

Serana, the vampire follower from Skyrim's Dawnguard DLC, has a level cap of 50, meaning her stats and abilities stop increasing once you reach that level, though she will scale up with you to that point; she's an essential follower who levels with the player but hits this limit, making her less effective at very high levels unless using mods. 
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Will Serana still follower if you choose Dawnguard?

Yes, you absolutely can get Serana as a follower if you join the Dawnguard; she's a core companion for the entire Dawnguard DLC questline, regardless of which faction (Dawnguard or Vampires) you ultimately side with, and remains a permanent follower after completion if you choose. She'll follow you through most of the main story as an essential guide, and after the questline, she'll stay at Fort Dawnguard (or Castle Volkihar if you chose vampires) and can be recruited indefinitely. 
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Can I be a Vampire Lord and join the Dawnguard?

Yes, you can become a Vampire Lord and still side with the Dawnguard in Skyrim: Dawnguard DLC, but you'll face challenges, as Dawnguard members become hostile and block quests until you're cured; you get chances from Serana in the questline after killing Harkon, allowing you to be a Vampire Lord and complete the Dawnguard side, then cure yourself for faction progression, and can be turned again later.
 
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What happens if I accept Harkon's gift?

Accepting Lord Harkon's gift in Skyrim's Dawnguard DLC instantly turns the Dragonborn into a Vampire Lord, aligning you with the vampires against the Dawnguard, giving you immense power but making you weak in the sun and blocking normal Dawnguard quests unless you get cured and later choose the Vampire path again via Serana. Refusing banishes you but leaves the choice open, and you'll be attacked on sight by vampires.
 
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How many times will Aela turn you into a Werewolf?

That's right, you can only become a Werewolf twice (with the Dawnguard DLC installed). Before Dawnguard, it was only once. In the words of Aela, Hircine doesn't usually bestow his favor on the fickle.
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Can Valerica turn you into a vampire?

Only Harkon and Serana can turn you into a Vampire Lord. Valerica has no such option.
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Can I turn Serana human?

Yes, you can turn Serana human in Skyrim by siding with the Dawnguard in the DLC and convincing her after defeating her father, Harkon, but you must choose specific dialogue options during the questline to make it possible; if you ask her about a cure too early or insult her, she'll refuse permanently, losing the ability to be cured later. Once cured, she loses vampiric abilities, and you lose the ability to become a Vampire Lord.
 
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Will Serana follow me if I'm not a vampire?

Serana is a pure-blood Vampire and follower who accompanies you throughout the Dawnguard questline. Her father is the vampire Lord Harkon. No matter which side you choose she still helps you.
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Can you still become a Vampire Lord if you refuse Harkon?

Yes, you absolutely can become a Vampire Lord even after refusing Harkon's offer; you just need to get turned by Serana later in the questline or after completing it, either during "Chasing Echoes," "Kindred Judgement," or as a follower, as long as she hasn't been cured of her vampirism. This allows you to get the Vampire Lord form and powers without siding with Harkon initially, though it might affect your relationship with the Dawnguard if you're actively helping them. 
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Is there a downside to becoming a vampire in Skyrim?

Being a vampire in Skyrim comes with severe drawbacks like massive penalties to health, magicka, and stamina in sunlight, extreme fire weakness, and being attacked on sight by NPCs at higher stages, forcing you to feed constantly or risk becoming a vulnerable, hated monster, which severely hinders exploration and social interaction unless you manage stages carefully. 
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