Who impregnated Iosefka in Bloodborne?

The imposter Iosefka in Bloodborne was impregnated by the Great One Formless Oedon, similar to Arianna, using a Third Umbilical Cord to beckon the cosmic entity for ascension, though her pregnancy manifests differently, leading to transformation rather than birth, notes this Fandom page and this GameFAQs discussion. She used a Willem's Cord to achieve this, revealing her transformation into a Kin-like being, and drops the Oedon Writhe rune, confirming her connection to Oedon, state this Reddit thread and this Fandom wiki entry.
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Should I kill the girl in Iosefka's clinic?

Killing her will make her drop the rune "Oedon Writhe," and her quest line will end. If the player causes her to become hostile and leaves, she will not be able to talk through the door again, even if the player sends more people to her clinic.
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Who is the imposter in Iosefka?

Imposter Iosefka is a minor antagonist in Bloodborne. Unlike the genuine Iosefka who seeks the safety of her patients from the Scourge plague, her imposter conducts them in experiments to further the evolution of humankind. She was voiced by Lucy Briggs-Owen.
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What happens if you kill Iosefka before ROM?

If you kill Iosefka in her Clinic before defeating Rom, the Vacuous, you get the Oedon Writhe Caryll Rune, but you miss out on her crucial Third Umbilical Cord, which is needed for the secret ending; however, you can still get other Umbilical Cords, so you likely won't lock yourself out of all endings, just the "secret" one unless you get the others. Killing her later (after Rom/Blood Moon) yields the cord instead of the rune. 
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Who is the real Iosefka?

The real Iosefka might be a member of the School of Mensis who had a change of heart. Leaving the Unseen Village she founded a clinic in the heart of Central Yharnam outside the spheres of influence of the Healing Church.
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Bloodborne Lore - Iosefka is an Impostor

Is Mergo Queen Yharnam's baby?

Mergo is an infant Great One, suspected to be the child of Yharnam, Pthumerian Queen and Oedon, a Great One. The child appears to have been stillborn in some fashion, as evidenced by the item description of a certain Third Umbilical Cord, "Every Great One loses its child, and then yearns for a surrogate.
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Did the Orphan of Kos create the nightmare?

Oooo, so Kosm was already dead, and it was the orphan who created the nightmare, got it.
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What happens if you get 40 insight in Bloodborne?

40 Insight:

Amygdalas can be seen hanging onto the Oedon Chapel in the Cathedral Ward and at the gates of Yahar'gul, without having to trigger the "Blood Moon" event.
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Can you keep Arianna alive Bloodborne?

Arianna can only be saved if she is met before triggering the Blood Moon. Otherwise, she will go mad and remain in her home.
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Does Bloodborne have NSFW?

Sex & Nudity

Statues of women without clothing are shown at Cainhurst Castle. No genitalia appear to be shown, nipples are shown on a couple. Some monsters appear nude and their rears exposed.
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Can you find Eileen after killing Rom?

If she survived the battle against Henryk she will be at the grand cathedral after killing rom. So go and kill rom then she will be there to kill you.
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Why does Gehrman hesitate to say his name?

Trivia. Gehrman seems to struggle to say his own name. Perhaps because he has been in the Hunter's Dream for a very long time.
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Who fathered the Orphan of Kos?

It's unknown how Kos became pregnant and who the father of Orphan was, but most likely he was born dead anyway because the Great Ones cannot have children and need a human surrogate.
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Is killing Eileen the Crow worth it?

Players can at any given point kill Eileen, but will only receive the Crow Hunter Badge. This will fail her questline, and players will receive nothing else. This means players will lose out on a special attire, two gestures, and a special Oath Memory Caryll Rune.
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Can you marry Queen Annalise?

The Ring of Betrothal unlocks a marriage proposal dialogue option when speaking to Annalise. However, she rejects the offer.
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What happens if the doll dies in Bloodborne?

The Doll can be killed and will revive after returning to the Hunter's Dream.
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Why is Lady Maria dead if she is in the Hunter's Nightmare?

Consumed by her guilt, Lady Maria eventually took her own life. In death, her consciousness became trapped within the Hunter's Nightmare, a twisted realm created from the curse of the Orphan of Kos.
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What if I consume four umbilical cords?

Nothing. The 4th is a failsafe to allow the player to get the ending they want. The umbilical cord you receive from Isoefka can be missed as you progress through the game.
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Is it worth killing Alfred in Bloodborne?

Killing Alfred

Killing him at his first location, at the church leading to Old Yharnam, causes him to drop three Fire Papers. Alfred will drop the Wheel Hunter Badge if killed before giving him the Unopened Summons. He will drop the Radiance Caryll Rune if he is killed at Forsaken Cainhurst Castle.
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Is 40 or 50 vitality better Bloodborne?

Practically speaking, a player should try to have between 40 and 50 Vitality to squeeze as much health value from the stat as possible. Along with Endurance, this is tied as the most important stat to level up.
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How many Insight does it take to see the amygdala?

In the game Bloodborne, you need 40 Insight to see the Lesser Amygdala creatures hanging in places like the Oedon Chapel and Yahar'gul before the Blood Moon event; otherwise, you need to defeat Rom the Vacuous Spider for them to become permanently visible, regardless of your Insight level. 
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Is there infinite Insight in Bloodborne?

It's unlimited. You get insight for defeating bosses as a summon, and you get multiple insight per chalice boss (you can create unlimited chalice dungeons).
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Is Rom a beast or a kin?

Rom is a Kin Great One. She was granted being a Great One by Kos, but at the same time, she is a Kin. She was beckoned by Willem to create a barrier, so humans couldn't see the eldritch secrets around them. Rom also appears to have more than one physical body, the second is in the Labyrinths under Yharnam.
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What's the most iconic Bloodborne quote?

Provost Willem: We are born of the blood, made men by the blood, undone by the blood.
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Why was Kos dead?

Whether or not they had a hand in the death of Kos is unknown, but most agree that the hunters did some blasphemous violation of her body, likely by dissecting her to get her eldritch blood.
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